Clinical Application of Immunofluorescence I. Grouping β-Hemolytic Streptococci

Thomas B. Smith
1965 Journal of Bacteriology  
SMITH, THOMAS B. (Armed Forces Institute of Pathology, Washington, D.C.). Clinical application of immunofluorescence. I. Grouping j3-hemolytic streptococci. J. Bacteriol. 89:198-204. 1965.-Procedures are described for the production of antistreptococcal serum in rabbits and for the preparation of group-specific conjugates for Lancefield groups A, C, and G. A modification of the conventional technique of absorption and inhibition to prevent cross-reactions with common antigens was used with
more » ... lent results. In addition, a promising new approach to eliminating cross-reactions of group A conjugate with antigens of groups C and G by dilution with group Avariant antiserum was tested. A complete method is introduced that enables the clinical laboratory to report whether group A streptococci are present in a given throat culture well within 24 hr after the physician collects the sample. on May 9, 2020 by guest
doi:10.1128/jb.89.1.198-204.1965 fatcat:pzq7i4pwgra3pkpt7enb23qrgq