Special issue in honour of Prof. Reto J. StrasserEfficacy of botanical pesticide for rotifer extermination during the cultivation of Nannochloropsis oculata probed by chlorophyll a fluorescence transient

L.T. ZHANG, R. XU, J.G. LIU
2020 Photosynthetica (Praha)  
Nannochloropsis is widely used in aquaculture as a feed source. However, large-scale cultivation of Nannochloropsis usually fails due to rotifer contamination. In order to identify an effective technique for reducing rotifer contamination, the effect of Brachionus plicatilis contamination on photosynthetic characteristics in Nannochloropsis oculata and the efficacy of the celangulin (CA):toosendanin (TSN) (1:9) combination for rotifer extermination were investigated using chlorophyll a
more » ... nce transient. B. plicatilis could directly devour microalgal cells and sharply reduced N. oculata density to very low levels. B. plicatilis also inhibited activities of PSII reaction centers, both acceptor and donor side, thereby damaging the photosynthetic performance of surviving N. oculata cells. However, the CA:TSN (1:9) combination could completely eliminate B. plicatilis, thereby preventing rotifers from devouring microalgae cells and protecting the photosynthetic performance of the surviving algal cells against rotifers damage. Therefore, the binary combination of CA:TSN (1:9), is considered to be a good candidate of botanical pesticide for controlling rotifer contamination. Abbreviations: ABS/RC -the specific energy fluxes for absorption; CA -celangulin; ET0/RC -the specific energy fluxes for electron transport; MAIP -maximal amplitude of fluorescence in the I-P phase; OJIP transient -chlorophyll a fluorescence transient; RCreaction center; RC/CS0 -the amount of active PSII RCs per excited cross section; TR0/RC -the specific energy fluxes for trapping; TSN -toosendanin; VJ -relative variable fluorescence at J-step; WK -relative variable fluorescence at K-step; δR0 -the efficiency of the reduction of end electron acceptors at the PSI acceptor side; φE0 -the quantum yield of electron transport.
doi:10.32615/ps.2019.164 fatcat:l7kha7hz4bhnfgklcucyh6sjge