A Mouse IgG1 Monoclonal Antibody Specific for N-Glycolyl GM3 Ganglioside Recognized Breast and Melanoma Tumors

Adriana Carr, Ailette Mullet, Zaima Mazorra, Ana Maria Vázquez, Mauro Alfonso, Circe Mesa, Enrique Rengifo, Rolando Pérez, Luis Enrique Fernández
2000 Hybridoma  
14F7 murine monoclonal antibody (MAb) is an IgG1 immunoglobulin that is generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins and in the presence of Freund's adjuvants. 14F7 MAb binds specifically to GM3(NeuGc), whereas neither N-glycolyl or N-acetyl gangliosides, nor a sulfated glycolipid, are recognized as assessed by enzyme-linked immunosorbent assay or immunostaining on thin layer chromatograms.
more » ... studies in fresh tumor tissues showed that 14F7 MAb strongly recognized in antigen expressed in human breast and melanoma tumors.
doi:10.1089/02724570050109639 pmid:10952412 fatcat:pniypbhu2vholnddvo4qhqfip4