Future of electrophoretic technology in clinical laboratory testing—From urinary protein electrophoretic analysis to urinary protein pathological analysis—

Ryo Kubota, Shiro Iijima, Kiyoko Shiba
2016 Electrophoresis Letters  
Cellulose acetate membrane (CAM) electrophoresis for protein fraction is an easy and simple method. We have classified urinary protein patterns into glomerular, tubular, and mixed, using the relative mobility of each band on CAM electrophoresis of urine samples from patients with different types of kidney diseases. To apply this classification method in the future, we analyzed urine samples from individuals with either orthostatic proteinuria or Dent disease. The samples from individuals with
more » ... thostatic proteinuria were analyzed using SDS-PAGE and detected specific 27.7 kDa and 39.2 kDa bands. In addition, when urine samples of 15 min and 30 min after lordotic load testing were analyzed using CAM electrophoresis, these protein fractionations showed glomerular injury pattern. CAM electrophoretic analysis of urine samples from patients with Dent disease showed a urinary protein fraction different from serum protein fraction. Slow α 2 and β fractions of this analysis were characterized. Furthermore, in urine from patients with Dent disease, cystatin C and prealbumin were detected using a natural contact blotting method. Prealbumin was detected in the slow α 2 fraction in addition to the prealbumin fraction. Subjecting urine sample of patients suffering from kidney diseases to an exhaustive analysis by CAM electrophoresis possibly is an extremely effective method in kidney disease diagnosis.
doi:10.2198/electroph.60.23 fatcat:w3ceoaadm5h6fps5tt637qbizu