Activity of human β-defensins 2 and 3 against ESBL-producing Klebsiella strains

Hany Sahly, Sabine Schubert, Jürgen Harder, Michael Kleine, Dorthe Sandvang, Uwe Ullmann, Jens M. Schröder, Rainer Podschun
2006 Journal of Antimicrobial Chemotherapy  
Objectives: To test the bactericidal activity of human b-defensins (hBDs) 2 and 3 against extendedspectrum b-lactamase (ESBL)-producing Klebsiella strains. Methods: Thirty-six Klebsiella pneumoniae and seventeen Klebsiella oxytoca ESBL-producing isolates from nosocomial infections were tested. The bactericidal activity of recombinantly synthesized hBD-2 and -3 was tested and the results were given either as lethal doses killing ‡90% of bacteria (LD 90 s) or as MBCs ( ‡99.9% killing). Results:
more » ... cept for one intermediately susceptible strain (MBC = 25 mg/L), all other ESBL-producing strains were highly susceptible to both defensins (LD 90 s and MBCs £ 12.5mg/L). Conclusions: The results underline the high efficacy of hBD-2 and -3 against ESBL-producing Klebsiella, making both defensins attractive candidates as antimicrobial agents to combat these increasingly troublesome bacteria.
doi:10.1093/jac/dkl003 pmid:16436542 fatcat:qf5du24yyzd4reurt2islfwpoe