Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)

Z.Z. Tian, F.Q. Zhang, Z.Y. Cai, S.L. Chen
2016 Genetics and Molecular Research  
Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the Illumina HiSeq 2000 platform. All microsatellite primers were tested on 48 P. cathayana individuals from four locations on the
more » ... locations on the Qinghai-Tibet Plateau. The observed heterozygosity ranged from 0.000 to 1.000, and the null-allele frequency ranged from 0.000 to 0.904. These microsatellite markers may be a useful tool in genetic studies on P. cathayana and closely related species.
doi:10.4238/gmr.15038406 pmid:27525845 fatcat:fwqg2yaevzhqtcqe3zxtwoaicu