EFFECT OF POMEGRANATE EXTRACT GALLIC ACID ON THE PROLIFERATION OF PROSTATE CANCER CELLS BY PROMOTING THE EXPRESSION OF IGFBP7

G.F. FAN, Z.G. YU, Y.B. LIANG, Z.G. XU, J. TANG
2020 Applied Ecology and Environmental Research  
Objective: Pomegranate extract gallic acid inhibits the proliferation of prostate cancer PC-3 cells by promoting IGFBP7 expression. Methods: PC-3 cells were divided into six groups: control group (NC), blank control group (BL), IGFBP7 overexpression group (IGFBP7), Pomegranate extract gallic acid group (5.0 μmol/L, 10.0 μmol/L, 20.0 μmol/L). Cell proliferation was detected by MTT assay; apoptosis of each group was analyzed by flow cytometry; the expression levels of IGFBP7, AKT and mTOR were
more » ... KT and mTOR were assessed by Western blot. Results: The proliferative plural of PC-3 cells in IGFBP7 group and pomegranate gallic acid group were significantly lower than those in NC and BL groups (P < 0.05). The pomegranate gallic acid group showed dose-dependence. The results of Western blot analysis showed that IGFBP7 group and The levels of IGFBP7 in the pomegranate extract gallic acid group were significantly higher than those in the NC and BL groups (P < 0.05), while the levels of AKT and mTOR plural were significantly lower than those in the NC and BL groups (P < 0.05). Pomegranate extract gallic acid showed dose-effect further elaborated within each group. Conclusion: Pomegranate extract gallic acid can inhibit the expression level of AKT/mTOR protein by enhancing the expression level of IGFBP7, thereby inhibiting the AKT/mTOR signaling pathway, the result is an inhibitory effect on the proliferation of prostate cancer PC-3 cells. Keywords: pomegranate extract gallic acid, IGFBP7 protein, AKT/mTOR protein, PC-3 cell, prostate cancer Fan et al.: Effect of pomegranate extract gallic acid on the proliferation of prostate cancer cells by promoting the expression of IGFBP7 -6240 -APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 18(5):6233-6241.
doi:10.15666/aeer/1805_62336241 fatcat:zgkeb3v3arhixn5gxtehdn5dpi