Identification of type D Pasteurella multocida by counterimmunoelectrophoresis

M M Chengappa, G R Carter, W E Bailie
1986 Journal of Clinical Microbiology  
A counterimmunoelectrophoresis (CIE) test was applied to serotype 35 isolates of type D Pasteurella multocida recovered from 32 cases of atrophic rhinitis (in swine) and 3 cases of snuffles (in rabbits). The CIE test was compared with the indirect hemagglutination (IHA) and acriflavine (AF) tests. Results of the CIE test correlated 100% with those of the IHA test whereas results of the AF test correlated 91.43% with those of the IHA test. The CIE test was rapid and simpler to perform compared
more » ... perform compared with the IHA test and more sensitive than the AF test. Cross-reactions were not encountered with capsular antigens of P. multocida types A, B, and E in the CIE test. The CIE test was not found to be suitable for typing type A P. multocida strains.
doi:10.1128/jcm.24.5.721-723.1986 fatcat:xhrogyocs5hshecglr5zoswooa