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Optical clearing agents can improve tissue optical transmittance by reducing the diffuse reflection. The reflection on in vivo human skin before and after applying anhydrous glycerol and 30 to 50% liquid paraffin glycerol mixed solution are investigated in this paper. From their visible and near-infrared reflection spectroscopy, all of their diffuse reflections are reduced after applying the agents. It is found that the three mixed solutions show stronger effect than that of anhydrous glycerol.doi:10.1117/1.jbo.18.10.105001 pmid:24091625 fatcat:mjjxqrvgpfhfrnrzvvnilo7eue