Application of rat medium-term bioassays for detecting carcinogenic and modifying potentials of endocrine active substances

Katsumi Imaida, S. Tamano, Akihiro Hagiwara, S. Fukushima, T. Shirai, Nobuyuki Ito
2003 Pure and Applied Chemistry  
Two in vivo bioassay methods, a rat medium-term liver bioassay and a rat multiorgan bioassay, can be used for detecting carcinogenic or modifying potentials of endocrine active substances (EASs) on endocrine disruption (ED). The first bioassay, the rat mediumterm liver bioassay, is fundamentally based on the two-step hypothesis of liver carcinogenesis; initiation with diethylnitrosamine (DEN, 200 mg/kg b.w., ip) is followed by test chemical administrations during the second stage, in
more » ... with 2/3 partial hepatectomy. It requires only eight weeks for animal experimental treatment and a further few weeks for quantitative analysis of immunohistochemically demonstrated gluthathione-
doi:10.1351/pac200375112491 fatcat:2zhi3mbsmvagli4lragmco2icm