We conducted a preregistered, multi-laboratory project (k = 36; N = 3531) to assess the size and robustness of ego depletion effects using a novel replication method, termed the paradigmatic replication approach. Laboratories implemented one of two procedures that intended to manipulate self-control and tested performance on a subsequent measure of self-control. Confirmatory tests found a non-significant result, d = 0.06. Confirmatory Bayesian meta-analyses using an informed prior hypothesis (δ

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... = 0.30; SD = 0.15) found the data were four times more likely under the null than the alternative hypothesis. Hence, preregistered analyses did not find evidence for a depletion effect. Exploratory analyses on the full sample (i.e., ignoring preregistered exclusion criteria; see supplemental online materials) found a statistically significant effect (d = 0.08), with data about equally likely under the null and informed prior hypotheses. Exploratory moderator tests suggested that the depletion effect was larger for participants reporting more fatigue but was not moderated by trait self-control, willpower beliefs, or action orientation.