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Deubiquitinating enzyme USP3 controls CHK1 chromatin association and activation
2018
Proceedings of the National Academy of Sciences of the United States of America
Checkpoint kinase 1 (CHK1), a Ser/Thr protein kinase, is modified by the K63-linked ubiquitin chain in response to genotoxic stress, which promotes its nuclear localization, chromatin association, and activation. Interestingly, this bulky modification is linked to a critical residue, K132, at the kinase active site. It is unclear how this modification affects the kinase activity and how it is removed to enable the release of CHK1 from chromatin. Herein, we show that the K63-linked ubiquitin
doi:10.1073/pnas.1719856115
pmid:29735693
fatcat:c2xuiedukrgzjlyjudaasnveve