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In Serbia, unambiguous symptoms of ash dieback disease were for the first time observed in September 2015. Symptoms included dead shoots and occasionally small necrotic lesions in the bark accompanied by characteristic wood discoloration. Isolation of fungal cultures from symptomatic tissues of F. excelsior and F. angustifolia and their sequencing using the internal transcribed spacer of the rDNA (ITS rDNA) as a marker confirmed the presence of the ash dieback pathogen, Hymenoscyphus fraxineus.doi:10.5281/zenodo.556518 fatcat:k7ghk3uzezhr3ilkebw4wyw5wq