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A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets
2020
Cell Reports
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We present a CRISPR-based multi-gene knockout screening system and toolkits for extensible assembly of barcoded high-order combinatorial guide RNA libraries en masse. We apply this system for systematically identifying not only pairwise but also three-way synergistic therapeutic target combinations and successfully validate double- and triple-combination regimens for suppression of cancer cell growth and protection against Parkinson's disease-associated toxicity. This system overcomes the
doi:10.1016/j.celrep.2020.108020
pmid:32783942
fatcat:hjxcaqy4cjcq7jkp2bb3silcj4