Antibacterial Activity of Ethanolic Extract and Infusion of Bauhinia variegata Leaves Against Streptococcus pyogenes Pengujian Aktivitas Antibakteri Ekstrak Etanol dan Infusa Daun Kupu-Kupu (Bauhinia variegata) Terhadap Bakteri Streptococcus pyogenes

Istiqamatush Sholihah, Ika Trisharyanti, Jl Yani, Tromol Pos, Pabelan Kartasura Surakarta
2017 PHARMACON   unpublished
Bauhinia variegata is a plant that has many benefits and widely grown in Indonesia. However, the utilization is still limited as the shade and ornamental plant. The purpose of this study was to determine the antibacterial ethanol extract activity and the infusion of B. variegata leaves toward Streptococcus pyogenes and to determine the classes of compounds that have antibacterial activity. B. variegata leaves were extracted by maceration and infusion with 96% ethanol and distilled water. The
more » ... t method of antibacterial activity was using disc difussion Kirby Bauer methods. The Analyses of the compounds contained in the B. variegata leaves were done by TLC (Thin Layer Chromatography) and phytochemical tests. The results showed that ethanol extract and infusion of B. variegata leaves did not have antibacterial activity toward Streptococcus pyogenes. TLC results and phytochemical testss showed that the ethanol extract of B. variegata leaves contained tannins, saponins, terpenoids, and alkaloids. Moreover, the infusion of B. variegata leaves comprised of saponins and alkaloids. Abstrak Tanaman kupu-kupu (Bauhinia variegata) merupakan tanaman yang mempunyai berbagai khasiat dan banyak ditanam di Indonesia. Daun kupu-kupu digunakan oleh masyarakat Sumba Barat Nusa Tenggara Timur sebagai obat bisul. Tujuan penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak etanol dan infusa daun kupu-kupu terhadapStreptococcus pyogenes dan mengetahui golongan senyawa yang mempunyai aktivitas antibakteri. Daun kupu-kupu diekstraksi dengan cara maserasi dan infundasi dengan pelarut etanol 96% dan akuades. Metode uji aktivitas antibakteri digunakan metode disc difusson Kirby Bauer. Analisis golongan senyawa yang terdapat dalam daun kupu-kupu dilakukan dengan cara Kromatografi Lapis Tipis (KLT) dan uji tabung. Hasil penelitian menunjukkanbahwa ekstrak etanol dan infusa daun kupu-kupu tidak mempunyai aktivitas antibakteri terhadap bakteri Streptococcus pyogenes. Hasil KLT dan uji tabung menunjukkan bahwa ekstrak etanol daun kupu-kupu mengandung tanin, saponin, terpenoid, dan alkaloid. Sedangkan infusa daun kupu-kupu terdapat saponin dan alkaloid.
fatcat:qeiaae7l4bhnbht434jf2w6ste