The elaboration of a practical protocol for the micropropagation of several apple rootstock varieties

Doina Clapa, Alexandru Fira, Manuela Simu, Monica Harta, Cristian Sisea
2016 Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture  
The apple rootstock varieties 'MM 106/4', 'MM 106/6', 'D 18', 'D 20', 'JTE-H' and 'MR 09/4' were multiplied in vitro on modified Murashige and Skoog media gelled with wheat starch (MSs) and supplemented with 0.7 mg/l BA, which provided intense axillary shoot proliferation. Among the genotypes we studied, MR 09/4 had the highest multiplication rate (19.56), followed by D18 (15.36). The lowest multiplication rates were recorded in MM 106/6 (5.36) and in MM 106/4 (3.32). The use of the technique
more » ... direct ex vitro rooting and acclimatization in floating perlite provided rooting percentages of more than 90 %.
doi:10.15835/buasvmcn-hort:12170 fatcat:3lzpk3cx5zh6lllnwjzylc7gdm