Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail

Diwesh Kumar Niraj, Pushpendra Kumar, Chinmoy Mishra, Raj Narayan, Tarun Kumar Bhattacharya, Kush Shrivastava, Bharat Bhushan, Ashok Kumar Tiwari, Vishesh Saxena, Nihar Ranjan Sahoo, Deepak Sharma
2015 Veterinary World  
D (2015) Single nucleotide polymorphism mining and nucleotide sequence analysis of Mx1 gene in exonic regions of Japanese quail, Veterinary World 8(12): 1435-1443. Abstract Aim: An attempt has been made to study the Myxovirus resistant (Mx1) gene polymorphism in Japanese quail. Materials and Methods: In the present, investigation four fragments viz. Fragment I of 185 bp (Exon 3 region), Fragment II of 148 bp (Exon 5 region), Fragment III of 161 bp (Exon 7 region), and Fragment IV of 176 bp
more » ... 13 region) of Mx1 gene were amplified and screened for polymorphism by polymerase chain reaction-single-strand conformation polymorphism technique in 170 Japanese quail birds. Results: Out of the four fragments, one fragment (Fragment II) was found to be polymorphic. Remaining three fragments (Fragment I, III, and IV) were found to be monomorphic which was confirmed by custom sequencing. Overall nucleotide sequence analysis of Mx1 gene of Japanese quail showed 100% homology with common quail and more than 80% homology with reported sequence of chicken breeds. Conclusion: The Mx1 gene is mostly conserved in Japanese quail. There is an urgent need of comprehensive analysis of other regions of Mx1 gene along with its possible association with the traits of economic importance in Japanese quail.
doi:10.14202/vetworld.2015.1435-1443 pmid:27047057 pmcid:PMC4774823 fatcat:5qwqfhhyv5entflmhkjwfy2vae