Fibronectin Fragments and the Cleaving Enzyme ADAM-8 in the Degenerative Human Intervertebral Disc

Nancy Ruel, Dessislava Z. Markova, Sherrill L. Adams, Carla Scanzello, Gabriella Cs-Szabo, David Gerard, Peng Shi, D. Greg Anderson, Marc Zack, Howard S. An, Di Chen, Yejia Zhang
<span title="">2014</span> <i title="Ovid Technologies (Wolters Kluwer Health)"> <a target="_blank" rel="noopener" href="" style="color: black;">Spine</a> </i> &nbsp;
Study Design-The presence fibronectin fragments (FN-fs) and the cleaving enzyme, A disintegrin and metalloproteinase domain-containing protein (ADAM)-8 were examined in human intervertebral disc (IVD) tissue in vitro. Objective-To investigate the presence and pathophysiological concentration of FN-fs and their cleaving enzyme, ADAM-8, in the human IVD tissue. Summary of Background Data- The 29kDa FN-f has been shown to result in extracellular matrix loss in rabbit IVDs. However, the
more &raquo; ... n of this biologically active fragment in the degenerative human IVD tissue has previously not been determined. Further, it is critical to identify the enzyme(s) responsible for FN cleavage in the IVD. Methods-Human degenerative IVD tissues were removed during spinal surgery. A normal appearing young adult and an infant human cadaveric sample were obtained as controls. Soluble proteins were extracted, and analyzed by Western blotting utilizing antibodies specific for the human FN neoepitope VRAA 271 . A purified 29 kDa FN-f was used to allow estimation of the
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1097/brs.0000000000000397</a> <a target="_blank" rel="external noopener" href="">pmid:25010013</a> <a target="_blank" rel="external noopener" href="">pmcid:PMC4229950</a> <a target="_blank" rel="external noopener" href="">fatcat:3ifh76vgqncwjflw4x3ne4h5gq</a> </span>
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