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Keywords Aflatoxin B1; Broilers; Lymphocytes; PicoGreen; Reactive oxygen species. ABStRACt The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B 1 (AFB 1 ) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB 1 concentrations in 5% CO 2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays weredoi:10.1590/1516-635x1603307-312 fatcat:5go3den7z5efdcsxyhmopw42ny