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Is the Glycolytic Flux inLactococcus lactisPrimarily Controlled by the Redox Charge?
Journal of Biological Chemistry
The involvement of nicotinamide adenine nucleotides (NAD ؉ , NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using 13 C and 31 P NMR to monitor in vivo the kinetics of the pools of NAD ؉ , NADH, ATP, inorganic phosphate (P i ), glycolytic intermediates, and end products derived from a pulse of glucose. Nicotinic acid specifically labeled on carbon 5 was synthesized and used in the growth medium as a precursor of pyridine nucleotides to allow for in vivo detectiondoi:10.1074/jbc.m202573200 pmid:12011086 fatcat:ivppk3uio5eozpupa6zon3nn34