DNA-guided genome editing using structure-guided endonucleases

Gaurav K. Varshney, Shawn M. Burgess
2016 Genome Biology  
The search for novel ways to target and alter the genomes of living organisms accelerated rapidly this decade with the discovery of CRISPR/Cas9. Since the initial discovery, efforts to find alternative methods for altering the genome have expanded. A new study presenting an alternative approach has been demonstrated that utilizes flap endonuclease 1 (FEN-1) fused to the Fok1 endonuclease, which shows potential for DNA-guided genome targeting in vivo.
doi:10.1186/s13059-016-1055-4 pmid:27640875 pmcid:PMC5025577 fatcat:es5jzx4gyrbxljfrjwcnpdm64i