25 Years ago in the Canadian Journal of Neurological Sciences

2000 Canadian Journal of Neurological Sciences  
Secondary epileptogenesis was induced in the hippocampal cortex of the paralyzed bullfrog by means of localized, unilateral, intermittent electrical stimulation (kindling). Stimuli were designed to yield a brief but definite afterdischarge. In control animals a progressive increase in after-discharge duration occurred at the 1° (stimulated) site and then at the 2° site (contralateral hippocampus). Spontaneous epileptiform potentials (SEPs) occurred between stimuli, eventually independently on
more » ... th sides. Cycloheximide (50mg/kg) caused 88-99% reduction in protein synthesis, measured by l4 Cleucine incorporation into brain protein. Cycloheximide-treated animals revealed no evidence of progressive prolongation of after-discharge duration when subjected to the kindling procedure (p=0.1205x10 7 ). SEPs were reduced in the cycloheximide-treated animals, and confined to 1° hemisphere (p=0.6xl0 10 ). Since cycloheximide did not disturb normal electrogenesis or disrupt the after-discharges, this experiment distinguishes processes dependent upon electrical events from those requiring macromolecular synthesis. Protein synthesis seems critical to the emergence of spontaneous and autonomous epileptiform behavior of neural aggregates. Can. J. Neurol. Sci. 1975;2:(Suppl 2):407 A-12 https://www.cambridge.org/core/terms. https://doi.
doi:10.1017/s0317167100120086 fatcat:i5cqh54bfjevth6p7aqdmwmi4a