The Origin of CD45-CD71+ Cells Enriched by MACS Technology

Masato Kantake
2018 Biomedical Journal of Scientific & Technical Research  
Fetal nucleated cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. An analysis of nuclear red blood cells (NRBCs) in pregnant woman is believed to be useful for understanding prenatal fetal status. However, the extreme scarcity of fetal cells puts strong demands on sensitivity and specificity. We have effectively used a simple MACS technology without density gradient centrifugation or lysis of RBCs [1]. Abstract Fetal nucleated cells within
more » ... cells within maternal blood represent a potential source of fetal genes obtainable by venipuncture. In our previous study, we have enriched CD45-CD71+ cells from maternal blood. To confirm the potential usefulness of our method, we analyzed the origin of the enriched CD45-CD71+ cells using a real-time polymerase chain reaction (PCR) system. The SRY (sex-determining region Y) gene was detected only in cells extracted from the blood of the pregnant woman with the male fetus. The relative dose of the SRY gene to the TFRC (transferrin receptor) gene was 0.001, indicating that 99.9% of the separated cells were from the mother and only 0.1% were of fetal origin. Our method using MACS technology was insufficient to analyze the fetal genome, methylome, or transcriptome, as some additional enrichment is needed, such as single cell technology Figure 1 : Flowcytometry of enriched CD45-CD71+ cells of patient 1. DNA was extracted from these CD45-CD71+ cells.
doi:10.26717/bjstr.2018.08.001648 fatcat:hkz3cclscnb3hgxziwyvemwej4