Objective: The aim of the present study is to evaluate the anti-ulcer activity of Ethanolic extract of Vitex negundo in pylorus ligation and aspirin induced gastric ulcers in rats. Methods: The antiulcer activity was studied in rats in which gastric ulcer was induced by oral administration of aspirin (200mg/Kg) and by pylorus ligation method .The animals used for the experiment were divided into 4 groups for each model, 6 rats for each group. In pylorus ligation model, all groups of rats were

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... e-treated with test drugs, Group-I(control) received 2%gum acacia-2ml/100g, Group-II (standard) received Ranitidine (60mg/kg.) and group-III, IV were treated with Ethanolic extracts 150mg/kg, 300mg/kg. respectively orally 30mins prior to pylorus ligation. The antiulcer activity of Vitex was assessed by determining and comparing gastric volume, free acidity, total acidity, pH, percentage of ulcer protection, , ulcer index. In aspirin induced ulcer model, all groups of rats were treated with test drugs for 7 days prior to Aspirin induced ulcer. Animals were divided into 4 groups and treated with drugs as in above model. After 7 days of treatment, animals were fasted for 24 hrs. Ulcers were produced by administration of Aspirin (200mg/Kg) on the day of sacrifice. The animals were sacrificed 4 hours later and stomachs were open along the greater curvature and ulcers were graded. Percentage of ulcer protection, ulcer index were observed and calculated. Results: The extract of Vitex negundo in pylorus ligation model, it decreased the ulcer index (1.66) and there was a decrease in total gastric acid and free acid (p<0.0001), and increases the pH value (p<0.0001) and also reduces the total gastric volume (p<0.0003), increases the percentage of ulcer protection (61.66%). In aspirin induced ulcer model, it decreases the ulcer index (10.66) and increases the ulcer protection (72.09%). Conclusion: The ethanolic extract of vitex negundo was clearly shows a protective effect against total acid, free acid, gastric volume and ulcer index and also increases pH and percentage of protection against ulcers in both models.