Synthesis and evaluation of bactericidal properties of Ag 2 O nanoparticles against Aeromonas hydrophila

S Shaffiey, S Shaffiey, M Ahmadi, Sayedeh Shaffiey
2015 Int. J. Nano Dimens   unpublished
The nanomaterials have important application in different field of science such as biology and pharmacology, which draws the attention of biologists towards this field of study more than before. As the worldwide mortality rate is high due to the pathogens and especially because of the bacteria associated Aeromonas and the antibacterial effect of metal nanoparticles is well known for centuries, these materials can be used to annihilate Aeromonas hydrophila. In this study, silver oxide
more » ... er oxide nanoparticles were synthesized by sol-gel procedure and antibacterial activity of silver oxide nanoparticles as a function of particle concentration against gram-negative bacterium Aeromonas hydrophila ATCC 7966T was carried out in liquid as well as solid growth media. Synthesized Ag 2 O nanoparticles (NPs) were characterized by X-ray diffraction (XRD), scanning electron micrograph (SEM) and transmission electron microscopy (TEM). The average size of the Ag 2 O NPs determined through transmission electron microscopy (TEM) 15 nm. The bactericidal effect of silver oxide nanoparticles was compared based on the diameter of inhibition zone in well diffusion tests in nutrient culture media. Minimum bactericidal concentration (MBC) of nanoparticles dispersed in peptone water, liquid cultures in 22-25 ºC for 24 h were determined.