Pectinases are one of the essential groups of enzyme and has application in various industries including food, textile, paper industry and waste management. Fruit wastes are one of the polluting solid wastes which has been utilized for the production of pectinase enzymes. Fruit wastes of orange, apple, banana, pineapple and grape which are good sources of pectin. A large group of pectinase enzyme that are capable of hydrolyzing the pectin are used in industrial sector to increase the yield and

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... larity of fruit juices. This study was undertaken with aim of isolating pectinase producing bacteria from rotten orange peel and applied on fruit juice clarification. In which rotten orange were collected from Kochi fruits market and subjected to laboratory activities. Isolation and screening for pectinase producing bacterial strains was done by spread method in pectinase screening agar media. Bacterial strains which produce clear zone around the bacterial colonies are identified as pectinase producers then they were subjected to microscopic and biochemical identification. Based on this process the isolated bacteria were identified as Gram-positive rod. About 0.4 unit of pectinase enzyme activity was quantified based on the DNSA method. In this study the production and optimisation studies revealed that Gram positive Bacillus requires 35 0 c, pH 5.5, pectin (carbon source), and peptone (nitrogen source) higher pectinase enzyme production. In the