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Although basal cells are known to function as human airway epithelial stem cells, analysis of these cells has been limited by in vitro culture techniques that only permit minimal cell growth and differentiation. Here, we report a protocol that dramatically increases the longterm expansion of primary human airway basal cells while maintaining their genomic stability using 3T3-J2 fibroblast co-culture and ROCK inhibition using Y-27632. We also describe techniques for the differentiation anddoi:10.1007/7651_2016_5 pmid:27539459 fatcat:4itwedvo3vbxfcrwgm5efovr3i