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Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres
[chapter]
2016
Msphere
Although basal cells are known to function as human airway epithelial stem cells, analysis of these cells has been limited by in vitro culture techniques that only permit minimal cell growth and differentiation. Here, we report a protocol that dramatically increases the longterm expansion of primary human airway basal cells while maintaining their genomic stability using 3T3-J2 fibroblast co-culture and ROCK inhibition using Y-27632. We also describe techniques for the differentiation and
doi:10.1007/7651_2016_5
pmid:27539459
fatcat:4itwedvo3vbxfcrwgm5efovr3i