Differential Genomics Output and Susceptibility of Iranian Pa-tients with Unilocular Hydatidose

Mahdi Mosayebi, Abdolhossein Dalimi Asl, Mohammad Moazzeni, Ghasem Mosayebi
The objective of this study was to investigate HLA-DRB1*and DQB1* allelic polymorphisms in Iranian patients with hydatidose. This is the first survey dealing with the correlation between HLA-DRB1* and DQB1* al-leles and cystic echinococcosis in Iranian patients. Methods: The study was carried out on 56 patients with confirmed cystic echi-nococcosis and 30 apparently healthy individuals living in Arak-Markazi Province by HLA-DRB1 and DQB1 typing through PCR-SSP method. The first step was to
more » ... st step was to identify the patients and blood sampling. DNA was prepared from whole blood and PCR-SSP with 31 primer mixes for per sample was used. PCR reaction mixtures were loaded in agarose gels and bands were observed under UV illumination and gel document after electrophoresis. Analysis of results was carried out with specific softwares and frequency and interpretation tables for calculation of P-value in χ2 test were provided via Fisher΄s exact test. Significant samples were analyzed by logistic regression and odds-ratios were calculated .