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Detection of neoplastic cells in blood of miniature pigs with hereditary melanoma
Tyrosine, a key enzyme of melanin biosynthesis, is widely used as a specific marker for the detection of dissemination or metastatic melanoma cells in peripheral blood and other tissues like lymph node or bone marrow, which are normally tyrosinase negative. The amplification of tyrosinase-specific mRNA by means of RT-PCR is a sensitive technique capable of detecting a single tumour cell in 5–10 ml of whole blood. We have utilised this method to analyse the peripheral blood of laboratorydoi:10.17221/7880-vetmed fatcat:xgf4xb6rfvgulozdyvz74ggsby