Optical vortex (OV) is light twisted around the propagation axis. Optical trap by OV exerts constant torque on a dielectric object in the same direction of angular momentum at any moment because OV has an orbital angular momentum. Recently, we have succeeded in manipulating beads three dimensionally using OV (Otsu et.al, Sci. Rep., 2014). In this study, we tried to demonstrate that OV trapping can be used for an analysis tool for biological rotary motors. As a result, DNA and F 1 -ATPase was

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... ipulated by OV via beads in a desired direction and speed. Escherichia coli cells tethered on a coverslip by a single flagellum was accelerated and decelerated by OV. These results show that OV has a potential to reveal the torque generation mechanism of molecular motors. 1P290 ビデオ計測による大腸菌の回転運動特性の統計解析 Statistical analysis of rotational motion properties of tethered E. coli by video measurement Most responses of organism to inputs are stochastic, and statistical analysis is important to discuss for biological behaviors (functions). Although it would be the best approach to perform large number of measurements with high accuracy, such approach is often not practical because of consumption of much time. When focused on statistical analysis, increasing the number of measurements even at low measurement precision is often effective in extracting the required information of target functions. Here, using video measurement, we quantitatively estimate rotational movement characteristics of tethered E. coli, as a model measuring samples. Under low special resolution conditions, we statistically extract information such as direction, speed, center, torque of rotation. 1P291 マイクロ波加熱効果:生体系に対する in situ マイクロ波照 射 NMR の応用 Microwave heating is widely used in the acceleration of organic reaction as well as activity enhancement of enzymes. However, detailed molecular mechanism of microwave heating effect has not well understood yet. It is especially important to understand microwave induced denaturation of lipid membrane and protein. We therefore newly developed in situ microwave irradiation NMR spectroscopy. 1 H NMR spectra of liquid crystalline samples were observed under microwave irradiation as a model of biological system. Chemical shift and line width was indicated that nonequilibrium local heating caused the liquid crystalline to isotropic phase transition. Moreover, we successfully raise the temperature of samples around 200 degree in a short time within the NMR probe. 1P292 走査型イオンコンダクタンス顕微鏡の単一細胞ナノバイオプ シーへの応用と細胞内 mRNA 局在性の評価 Single-cell nanobiopsy to investigate intracellular mRNA localization using scanning ion conductance microscopy The cellular function is controlled by temporal and spatial changes in gene expression. Recent advances in qPCR technology make it possible to obtain information on single-cellular transcriptome, but the methods to quantify mRNA localization in single cell are still limited. Here we introduce the single-cell nanobiopsy using scanning ion conductance microscopy (SICM) technique for analysis of intracellular mRNA localization. For nanobiopsy platform, electrowetting in glass nano-pipette which enable the fL-pL extraction was used. By adopting the SICM technology, the positioning of nano-pipette was precisely controlled and the information of cellular topography could be obtained. We'll introduce the result of quantitativity of this system and application of living cell. 1P293 高速 AFM を用いたタンパク質構造変化のライブイメージ ング The trp-RNA binding attenuation protein (TRAP), from Bacillus stearothermophilus, is a ring shaped protein composed of 11 subunits. A lysine residue, located at the outer surface of the ring, was mutagenized to cysteine forming TRAP-K35C. After adding 1.4 nm diameter gold nanoparticles (GNPs) to TRAP-K35C, the thermostable ring structure transformed into a larger hollow sphere. Of significance is the addition of reducing agents which cause the hollow sphere to revert back to the original ring structure. In this study, high-speed atomic force microscopy (HS-AFM) was used to observe the dynamics of sphere disassembly. Insights into the sphere structure, mechanism of assembly/disassembly, and structural intermediates will be discussed. 1P294 マニフォールドの概念に基づく新規画像分類法を用いた投影 イメージの解析 Analysis of the projection images using the novel classification protocol based on the concept of manifold We have recently proposed a novel classification protocol of projection images toward applications to the data obtained through coherent X-ray diffraction imaging (CXDI) experiments. The protocol is based on the concept of manifold, and can be applied to projection images involving those generated from the particle conformations with a subtle difference as well as those with a large difference. Such projections would be obtained through CXDI experiments. In the present study, we apply the classification protocol to the projection images generated on a computer. We also analyze the classified data in terms of the projection direction (the Euler angle). We are going to discuss usefulness of the protocol through the application and the analysis. -S189 -Poster, Day 1