Analysing quantitative data from correlative microscopy techniques is of increasing interest to the research community, with many more imaging modalities being incorporated into correlative workflows [1]. Recording dynamic changes using more than one microscopy technique is also a significant challenge, involving reimaging the same region more than once with any chosen modality. We have used correlative microscopy to record changes in tooth enamel structure, chemistry, mechanical properties,

more »

... topography following exposure to acidic solutions using electron microscopy, energy dispersive x-ray spectrometry (EDS) and atomic force microscopy (AFM), respectively.