Activation of the Kallikrein-Kinin System in Human Plasma by a Serine Protease from Mites

Shoichi KOHMOTO, Yoh KODERA, Katsunobu TAKAHASHI, Hiroyuki NISHIMURA, Ayako MATSUSHIMA, Yuji INADA
1991 Journal of clinical biochemistry and nutrition  
A serine protease was earlier purified to homogeneity from a culture extract of mites and its possible involvement in the allergic reaction was proposed [K. Takahashi et al. (1990) : Int. Arch. Allergy Appl. Immun., 91, 80-85]. The serine protease thus obtained was found in the present study to be trypsin-like protease and had high substrate specificity towards the synthetic substrate for blood coagulation factor XIIa. The enzyme gave rise to rhythmic contraction of rat uterine horns in factor
more » ... II-deficient human plasma, but not in prekallikrein-deficient plasma. These findings lead us to conclude that this enzyme activates the kallikrein-kinin system in plasma through hydrolysis of prekallikrein in the plasma.
doi:10.3164/jcbn.10.15 fatcat:hagkqcjc25di7jzxp4iv2h75cq