d-Alanine Oxidase from Escherichia coli: Localization and Induction by l-Alanine

R. P. Raunio, W. T. Jenkins
1973 Journal of Bacteriology  
Dialyzed membranes of Escherichia coli prepared by an ethylenediaminetetraacetic acid-lysozyme method catalyze the oxidation of both L-alanine and D-alanine. The specific activities for the oxidations of both D-alanine and L-alanine are increased fivefold when the cells are grown in the presence of either L-alanine or DL-alanine, but are increased only slightly when grown in the presence of D-alanine. In the DL-alanine-induced system, the specific activities for the oxidations of some other
more » ... s of some other D-amino acids are also raised. DL-alanine also induces two other alanine catabolizing enzymes, alanine dehydrogenase and alanine-glutamate aminotransferase which are found in the "soluble" fraction of lysozyme-treated cells. The oxidations of both L-alanine and D-alanine were associated with the membranes of induced cells. After the membranes were disintegrated by sonic treatment, both L-alanine and D-alanine oxidation catalysts sedimented in a sucrose density gradient together with D-lactate and L-lactate dehydrogenases, apparently as a single multienzyme complex. Stephenson and Gale pioneered in studying factors which influence bacterial deaminations
doi:10.1128/jb.115.2.560-566.1973 fatcat:zo4lkxrab5cfhizhilw7d3yqo4