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Carotenoid uptake and secretion by CaCo-2 cells
Journal of Lipid Research
In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble and secrete chylomicrons. Under these conditions, both cellular uptake and secretion into chylomicrons of ␤ -carotene ( ␤ -C) were curvilinear, time-dependent (2-16 h), saturable, and concentration-dependent (apparent K m of 7-10 M) processes. Under linear concentration conditions at 16 h incubation, the extent of absorption of all-trans ␤ -C was 11% (80% in chylomicrons), whiledoi:10.1194/jlr.m200068-jlr200 pmid:12091493 fatcat:wf7nq5vnhbelrhzldsow56yqsm