An optimized toolkit for precision base editing [article]

Maria Paz Zafra, Emma M Schatoff, Alyna Katti, Miguel Foronda, Marco Breinig, Anabel Y Schweitzer, Amber Simon, Teng Han, Sukanya Goswami, Emma Montgomery, Jordana Thibado, Francisco J Sanchez-Rivera (+5 others)
2018 bioRxiv   pre-print
CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single base pair resolution. By re-engineering both DNA and protein sequences, we developed a collection of constitutive and inducible base editing vector systems that dramatically improve the ease and efficiency by which single nucleotide variants can be created. This new toolkit is effective in a wide range of model systems and provides a means for efficient in vivo somatic base editing.
more » ... somatic base editing.
doi:10.1101/303131 fatcat:vtnbfqu5fvbhvcavlbpxr3cj4i