Artifact Formation in Single Molecule Localization Microscopy [article]

Jochen Michael Reichel, Thomas Vomhof, Jens Michaelis
2019 bioRxiv   pre-print
We investigate the influence of different accuracy-detection rate trade-offs on image reconstruction in single molecule localization microscopy. Our main focus is the investigation of image artifacts experienced when using low localization accuracy, especially in the presence of sample drift and inhomogeneous background. In this context we present a newly developed SMLM software termed FIRESTORM which is optimized for high accuracy reconstruction. For our analysis we used in silico SMLM data
more » ... compared the reconstructed images to the ground truth data. We observe two discriminable reconstruction populations of which only one shows the desired localization behavior.
doi:10.1101/700955 fatcat:w4ghuyd6tfgvbbuhdzbegzr6g4