The impact of vanadium on endothelial dysfunction in type 2 diabetic rats: Histological insight

Mohamed Haidara, Abbas El Karib, Ismaeel Bin-Jaliah, Mohamed Morsy, Abdullah Shatoor, Salah Bashir, Basiouny El-Gamal, Mohammed Kemeir, Refaat Eid
2014 Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology  
The aim of this research is to investigate the effect of vanadium and or insulin on endothelial dysfunction in type 2 diabetic rats (T2DM). Material and methods:60 white albino rats were included in this research and randomly divided into 6 groups(n=10) as follows: Control group (Control): rats injected once intraperitoneally (i.p.) with citrate buffer (0.1 M, pH 4.5), vanadium treated group (Vanadium): rats received vanadyl sulfate of 0.64 mmol/kg weight freshly dissolved in 1 ml of distilled
more » ... ater daily through a nesophageal tube, diabetic type 2group(D type 2) : rats received high fat diet for 15 days followed by streptozotocin (25 mg/kg body weight ), diabetic type 2 and insulin group (D type 2 +I): D type 2 rats received mixtard insulin subcutaneously in a dose of 0.75 IU/100 gm weight in 0.75 ml volume once daily, diabetic type 2 and vanadium group (D type 2+V): type 2 diabetic ratsreceived the same dose of vanadium as in vanadium group after 48 h of induction of type 2 diabetes, diabetic type 2 + insulin + vanadium group (D type 2 +V+I): diabetic type 2 rats received both vanadium and insulin with the same doses as in previous groups. Results: Diabetic group showed hyperglycemia, increased cholesterol(C), triglycerides (TG), LDL-C and decrease HDL-C. Administration of both insulin and vanadium caused significant decrease of glucose, TG, C, LDL-C and increased HDL-C back to control level. Histological analysis of aortae documented our results. Conclusion: Administration of vanadium enhances the effect of insulin and restore endothelial damage in T2DM.
doi:10.21608/eajbsc.2014.16049 fatcat:konftkkporh47cybb74rqpodyu