Genomics-enabled sensor platform for rapid detection of viruses related to disease outbreak [report]

Susan M Brozik, Ronald P Manginell, Matthew W Moorman, Xiaoyin Xiao, Thayne L. Edwards, John Moses Anderson, Kent Bryant Pfeifer, Darren W. Branch, David Roger Wheeler, Ronen Polsky, DeAnna M. Lopez, Gregory D. Ebel (+4 others)
2013 unpublished
Bioweapons and emerging infectious diseases pose growing threats to our national security. Both natural disease outbreak and outbreaks due to a bioterrorist attack are a challenge to detect, taking days after the outbreak to identify since most outbreaks are only recognized through reportable diseases by health departments and reports of unusual diseases by clinicians. In recent decades, arthropod-borne viruses (arboviruses) have emerged as some of the most significant threats to human health.
more » ... s to human health. They emerge, often unexpectedly, from cryptic transmission foci causing localized outbreaks that can rapidly spread to multiple continents due to increased human travel and trade. Currently, diagnosis of acute infections requires amplification of viral nucleic acids, which can be costly, highly specific, technically challenging and time consuming. No diagnostic devices suitable for use at the bedside or in an outbreak setting currently exist. The original goals of this project were to 1) develop two highly sensitive and specific diagnostic assays for detecting RNA from a wide range of arboviruses; one based on an electrochemical approach and the other a fluorescent CONTENTS ABSTRACT .
doi:10.2172/1096502 fatcat:3qhdorybm5bongk56fcyn4t5kq