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Additional file 1: Figure S1. of Sirt1 interaction with active Smad2 modulates transforming growth factor-β regulated transcription
TGFβ induces the acetylation of Smad2. (a) Hep3B cells were serum starved overnight, treated with TSA for 8 h followed by TGFβ for the indicated times. Smad2 was purified by immunoprecipitation, then acetyl-Lys,Smad2, Smad4 and PSmad2 were detected by Western Blot. Ack/Smad2 fold increase is indicated. (b) Schematic representation of Smad2 (K19R, K20R, K39R and K19Q, K20Q, K39Q) acetylation mutants. (c) Schematic representation of Sirt1H363Y nucleotide mutation. Nucleotide and amino acid mutations are highlighted in red. (PDF 219 kb)doi:10.6084/m9.figshare.5648797.v1 fatcat:nusmqqzr7zfxdmlbesljfuylvi