Enterobacter cloacae inhibits human rotavirus infectivity in vitro

Mohamed NF Shaheen, Neveen M Rizk, Abdou K Allayeh, Samy M Abdelhamid, Elmahdy ME Ibrahim
2019 International Journal of Molecular Biology  
Rotavirus (RV) is a major agent of acute gastroenteritis in human worldwide. Currently no efficient drug to inhibit RV gastroenteritis and vaccines remains the only available strategy to prevent and control RV infections. This study aimed to investigate the effect of Enterobacter cloacae on RV infections in vitro. For this purpose, the cytotoxic effect of this bacterium on cell culture was tested then the antiviral assay was conducted using different concentrations of E. cloacae and strategies
more » ... cae and strategies to understand the mechanism by which E. cloacae can inhibit RV infections. Our results demonstrated that E. cloacae was safe to cells at concentration up to 1.00E+0 6 CFU/ml. The Highest antiviral activity was observed from E. cloacae at concentration 1.00E+0 6 CFU/ml when the cell lines were treated with the E. cloacae plus RV together at the same time (competition assay) and when the cell lines treated with E. cloacae before RV infection (pre-treatment assay), protecting 62% and 55% of cell line, respectively, with reduction in virus titers by 1.5 log 10 TCID 50 /ml and 1.3 log 10 TCID 50 /ml, respectively. Our findings revealed the potential of E. cloacae in inhibition of rotavirus infections.
doi:10.15406/ijmboa.2019.04.00112 fatcat:xmal2kkeqjewxmlw62chcs7pne