Assessment of Total Lipid Fractions and Fatty Acids Composition in Raw, Germinated Barleys and Talbina Products
Food and Public Health
Talbina is a food product with high potential applications as a functional food. Talbina was prepared from two barley varieties namely: Giza126 and Giza130 by adding whole barley flour to water (1:10 w/v) and (1:5 w/v) for germinated barley then heating at 80° C for 5 minutes with continuous stirring until reaching a porridge like texture. The study included the fractionation and determinations of the compounds of raw, germinated barley, talbina, germinated talbina and commercial talbina oil.
... cial talbina oil. Besides, the all treatments recorded rather slight decrease in crude fat content, which ranged from 1.5 to 2.9%. Using TLC technique the total lipids of raw, germinated barley and their talbina products were fractionated to eight fractions and triglycerides showed the highest percentage among lipid fractions (33.92-60.82%) followed by 1,3diglycerides (6.78-20.43%). The fatty acids analysis revealed that there were 11 fatty acids in the studied treatments oil namely: caprylic, lauric, myristic, palmitic, stearic, arachidic, oleic, linoleic, linolenic and gadoleic. The essential fatty acid linoleic (C18:2) recorded the highest percentage of the unsaturated fatty acids (53.59%) in germinated 130. While palmitic acid (C16:0) recorded the highest value of saturated fatty acids in germinated 126 (17.44%). classified into two basic fractions: nonstarch lipids and starch lipids. Nonstarch lipids include all lipids other than those inside starch granules. Lipids are also classified as polar or non polar, a characteristic that affects solubility and depends on molecular structure (Rosemary et al., 2008) . Lipid is concentrated in the embryo, and although the embryo represents only about 3% of the kernel by weight, it furnishes about 18% of the total lipid in barley, while the endosperm contains about 3% lipid, but by virtue of size, furnishes about 77% of the total lipid, Most of the endosperm lipid is found in the aleurone layer. The remaining 5% of the total kernel lipid is found in the hull Parsons 1975, 1979; Briggs 1978) . Furthermore, Erkan et al., (2006) reported that fat ranged between 1.62-1.92% in hulled barley and 1.9% in hull-less barley. Similar results were shown by Welch (1978) , who found that oil content ranged from 1.9 to 4.1% and represented positive correlation with protein content. Price and Parsons, (1979) studied the composition of fatty acids from hulless barley and their research revealed that linoleic acid was the predominant unsaturated fatty acid and palmitic acid was the major saturated fatty acid of the neutral lipids, phospholipids and glycolipids, which is in agreement with our results. Morrison (1993a) also noted that the fatty acids in barley are similar to those in wheat except that barley tends to