The Clastogenic Potential of Triazine Herbicide Combinations Found in Potable Water Supplies

Carrie Taets, Susanne Aref, A. Lane Rayburn
1998 Environmental Health Perspectives  
Pesticide contamination of drinking water supplies has increased over the past decade. A major concern is how exposure to combinations of low levels of pesticides, especially herbicides, could affect public health. Flow cytometric analysis was performed to determine the dastogenic potential of herbicide interaction on Chinese hamster ovary (CHO) cells. The cells were exposed to atrazine, simazine, cyanazine, and all possible combinations of these chemicals for 48 hr. Two concentrations were
more » ... for each sample: the U.S. EPA maximum contmination level (MCL) and the highest contamination level found in Illinois water supplies. Nuclei were isolated from the cells and analyzed by flow cytometry. The efects of dastogenicity were measured by the coefficient of variation (CV) of the Gi peak of whole cells and the change in CV of the largest chromosome in the flow karyotype. At both levels tested, atrazine caused chromosomal damage to the CHO cells. Simazine was observed to induce whole-ell clastogenicity but not flow karyotype damage. Cyanazine did not induce any measurable chromosomal damage in either analysis. Each of the herbicides, although all three were triazines, had di&Terent efcts with respect to
doi:10.2307/3433963 fatcat:icfyb5ie3jdqvjl54ntxt5pzwa