Bacterial Isolates from Seafood in Scotland

NgoziIzuchukwu NgoziIzuchukwu
2017 IOSR Journal of Pharmacy and Biological Sciences  
Vibrio spp., Enterobacteriaceae representatives and Listeria monocytogenes were isolated from commercially prepared smoked and fresh Atlantic salmon, smoked and fresh haddock, live mussels and oysters. Isolates were identified phenotypically and by sequencing the 16S rDNA gene. Vibrio spp. occurred in high densities (>10 6 CFU gˉ1) in mussels. Enterobacteriaceae representatives were recorded at 2.2 x 10 6 CFU gˉ1 and 2.0 x 10 6 CFU gˉ1in fresh salmon and smoked haddock, respectively. Total
more » ... ctively. Total heterotrophic counts in fresh salmon, live mussels and oysters reached 10 7 , 10 7 and 10 6 CFU gˉ1, respectively. Listeria monocytogenes was recorded at 5.0 x 10 4 CFU gˉ1in mussels. The prominent characteristics of fish spoilage isolates were demonstrated by the ability of the isolates to reduce trimethylamine oxide (TMAO) to trimethylamine, and to produce H₂S. The spoilage organisms revealed the ability to produce hydrolytic enzymes.
doi:10.9790/3008-1202042835 fatcat:dz3b7maumvfufdr45h4df4atw4