Histomorphological study of gastric mucosal biopsy in dyspepetic patients and its association with Helicobacter pylori infection

Prachi ., M Shanthi, Kasa Somsekhar
2020 Eastern Journal of Medical Sciences  
The rationale of the study was to evaluate the morphological changes of chronic gastritis and its association with Helicobacter pylori infection and the efficacy of hematoxylin and eosin (H & E) and Giemsa techniques in a limited set up which helps in providing the vital data for subsequent treatment regimen. Materials and Methods: In this prospective study, 50 endoscopic biopsies were obtained from dyspeptic patients from January 2016 to June 2017. The samples were fixed in 10% buffered
more » ... n and histopathological slides were prepared using paraffin blocks and subsequently stained with routine H & E and special stain with Giemsa. Results: There was a male preponderance in the study with a ratio of 2.3:1; mean age of patients was 37.59 years. Of the 50 patients, 47 patients (94%) had chronic gastritis. Twenty-one out of 47 samples showed positivity for H. pylori with routine H & E staining, which was confirmed with modified Giemsa staining method. Conclusion: Our study highlights the association of H. pylori in patients with functional dyspepsia and demonstrate the importance of cost effective Giemsa staining which can be practically applicable in Indian set-up for the detection of H. pylori.
doi:10.32677/ejms.2020.v05.i04.004 fatcat:eebmfiy2y5f65nydbyzomo3r5m