HSA and CA-125 Binding Study of [Pr-(DO3-Ch-Ph-Am-Gd(III))2Pt(IV)] Complex as M-MRI Contrast Agent for Ovarian Cancer Treatment

E.Francy Irudaya Rani T. Lurthu Pushparaj
2021 Zenodo  
The elevated polar nature of the Hetero trinuclear Gd(III)-Pt(IV) complex due to the existence of hydrophilic coumarone –phenyl amide pendant arm shows incredible enhancement in relaxivity (r1p = 77.68 mM-1 s-1 and r2p = 127.36 mM-1 s-1) when bonded with Human Serum Albumin in PBS buffer. The binding of the complex with HSA protein form an aduct and supports the relaxivity enhancement by reducing the molecular rotation correlation time due to the increase in molecular weight. The r2p/r1P ratio
more » ... The r2p/r1P ratio confirms that the complex is a T1-weighted contrast agent this is similar with that of the complex in neat aqueous solution. The complex also exhibits pH dependant relaxivity at different solution pH from 2.6 to 10.6. The pH dependence T1 and T2 values are in the range r1p= 85.36 to 7.89, r2p = 143.18 to 28.45, respectively, which confirms the suitability of the complex for cancer tissue analysis. The MTT assay study on the complex confirms the excellent anticancer activity of the complex against HeLa cells. The IC50 value of the complex is 10.98 μM, this higher value for the complex indicates the superior cytotoxic action against the HeLa cell lines.
doi:10.5281/zenodo.4592844 fatcat:ghc6dyp2nvc2dmhshlr2u6247q