Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings [chapter]

Estelle Remy, Paula Duque
2016 Msphere  
8 Heavy-metal soil contamination is one of the major abiotic stress factors that, by 9 negatively affecting plant growth and development, severely limit agricultural 10 productivity worldwide. Plants have evolved various tolerance and detoxification 11 strategies in order to cope with heavy-metal toxicity, while ensuring adequate supply 12 of essential micronutrients at the whole-plant as well as cellular levels. Genetic 13 studies in the model plant Arabidopsis thaliana have been instrumental
more » ... been instrumental in elucidating 14 such mechanisms. The root assay constitutes a very powerful and simple method to 15 assess heavy-metal stress tolerance in Arabidopsis seedlings. It allows the 16 simultaneous determination of all the standard growth parameters affected by heavy-17 metal stress (primary root elongation, lateral root development, shoot biomass and 18 chlorophyll content) in a single experiment. Additionally, this protocol emphasizes the 19 tips and tricks that become particularly useful when quantifying subtle alterations in 20 tolerance to a given heavy-metal stress, when simultaneously pursuing a large 21 number of plant lines, or when testing sensitivity to a wide range of heavy metals for 22 a single line. 23 24 2
doi:10.1007/978-1-4939-3356-3_16 pmid:26867625 fatcat:nyucetgp7rbh7o7wdn5g3t3opm