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Protein Kinase C Expression Is Increased upon Differentiation of Human Skeletal Muscle Cells: Dysregulation in Type 2 Diabetic Patients and a Possible Role for Protein Kinase C in Insulin-Stimulated Glycogen Synthase Activity

C. E. Chalfant
2000 Endocrinology  

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Protein kinase C (PKC) is a key enzyme in regulating a variety of cellular functions, including growth and differentiation. PKC is the most abundant PKC isoform expressed in skeletal muscle; however, its role in differentiation and metabolism is not clear. We examined the effect of muscle cell differentiation on PKC expression in human skeletal muscle cells from normal and type 2 diabetic subjects. Low levels of PKC messenger RNA (mRNA) and protein were detected in human myoblasts from both
more » ... s of subjects. Upon differentiation into myotubes, PKC mRNA and protein were increased 12-fold in myotubes from normal subjects. In human skeletal muscle cells obtained from type 2 diabetic subjects, increases in PKC mRNA and protein were not observed upon differentiation into myotubes al-
doi:10.1210/en.141.8.2773 pmid:10919262 fatcat:kx57nktxbbgthludfdj3kois2q
Citation

C. E. Chalfant. "Protein Kinase C Expression Is Increased upon Differentiation of Human Skeletal Muscle Cells: Dysregulation in Type 2 Diabetic Patients and a Possible Role for Protein Kinase C in Insulin-Stimulated Glycogen Synthase Activity." Endocrinology (2000) 2773-2778