THE SEPARATION AND QUANTITATIVE DETERMINATION OF CIPROFLOXACIN IN A PHARMACEUTICAL FORMULATION BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPH
Objective: A rapid, selective and sensitive ultra performance liquid chromatography method for quantitative determination of ciprofloxacin in pharmaceutical formulation has been developed and validated. Methods: The assay of ciprofloxacin was carried out by using an octadecyl (C18) with particle size 1.8µm column connected with Waters Acquity UPLC system. A buffer solution was prepared by diluting 2.9ml of phosphoric acid (85%) with 1000ml of water and adjusted to p H 3.0 with triethylamine.
... h triethylamine. The mobile phase was prepared by mixing the buffer and acetonitrile in the ratio of 88:12 v/v respectively. The flow rate was set as 0.3ml minute-1 , column oven temperature at 50°C and detection was made at wavelength 278nm. Results: The % RSD of peak areas from five replicates of ciprofloxacin was found to be 0.18, the tailing factor and theoretical plates for ciprofloxacin were found to be 1.20, 17341 respectively. The Resolution between ethylenediamine and ciprofloxacin was found to be 10.41. The linearity of detector response was noticed in the concentration range 6.33µg mL-1 to 50.69µg mL-1 of ciprofloxacin and the coefficient of variation was obtained as 0.9992. Conclusion: The obtained results evident that the method is precise and accurate and hence the proposed method is suitable and can be employed for assay of ciprofloxacin in pharmaceutical formulations.