Effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes

Dipannita Baishya, Arundhati Bora, J Goswami, Aunbha Baruah, DJ Dutta, Dr. Champak Barman, Shantanu Tamuly
2020 Journal of Entomology and Zoology Studies  
The present experiment was conducted to investigate the effect of fetal bovine serum on in vitro maturation and fertilization of bovine oocytes. The mean percentages of in vitro maturation performance of bovine oocytes based on cumulus cells expansion in both serum and serum free basic maturation media in type-I & type-II ovaries were recorded as 76.52 ± 3.95 and 64.81+ 4.60 & 73.33 ± 5.71 and 62.96 ± 6.57 respectively. The mean percentages of in vitro maturation based on cumulus cells
more » ... were recorded as 75.43 ± 3.25 and 64.20 ± 3.77 respectively in serum and serum free culture media irrespective of ovarian types. So, the mean percentage of in vitro maturation performance of bovine oocytes based on cumulus cells expansion increased significantly (P< 0.05) in serum basic maturation media than in serum free media in both type I and type II ovaries and also irrespective of ovarian types. The mean fertilization rates (%) were found to significantly higher (P< 0.05) in serum culture media (70.33 ± 3.21) than the serum free media (55.81 ± 4.33). The mean cleavage percentages recorded from 4-cell to 16-cell stages of embryos in serum culture media were found to be significantly higher (P< 0.05) than the serum free media.
doi:10.22271/j.ento.2020.v8.i5ad.7802 fatcat:zgjcu3rnz5bohdq6legmx5kvfm