Ultrastructural Morphological Alterations during Hyperoxia Exposure in Relation to Glutathione Peroxidase Activity and Free Radicals Productions in the Mitochondria of the Cortical Brain

Ismaeel Bin-Jaliah, Al-Said Haffor
2018 International Journal of Morphology  
BIN-JALIAH, I. & HAFFOR, A. S. Ultrastructural morphological alterations during hyperoxia exposure in relation to glutathione peroxidase activity and free radicals productions in the mitochondria of the cortical brain. Int. J. Morphol., 36(4):1310Morphol., 36(4): -1315Morphol., 36(4): , 2018 SUMMARY: Exposure to normobaric hyperoxia (NH) is known to increase the production of reactive oxygen species (ROS) by mitochondria. The present study was designed to examine mitochondrial ultrastructure
more » ... l ultrastructure morphological changes in the cortical brainin relation to glutathione peroxidase (GPX) activity and free radicals (FR) productions in brain tissue during hyperoxia exposure. The experimental groups were exposed to NH for 24 and 48 h continuously. Following the exposure periods, animals were sacrificed and cortical tissues were divided randomly into two parts; the first part was processed for the ultrastructural examination and the second was homogenized for GPX and FR determinations. Analysis of variance (ANOVA) showed that the main effects of O2 exposure periods were significant (p<0.05) for GPX and FR. Pair-wise means comparisons showed that NH elevated the average (+SE) GPX activity significantly (p<0.05) from the baseline control value of 5670.99+556.34 to13748.42+283.04 and 15134.19+1529.26 U/L with increasing length of NH exposure period from 24 to 48 h, respectively. Similarly, FR production was increased significantly (p<0.05) to 169.73+10.31 and 185.33+21.87, above baseline control of 105.27+5.25 Unit. Ultrastructure examination showed that O2 breathing for 48 h resulted in giant and swelled mitochondria associated with diluted inner membrane and damaged cristae. These mitochondria pathological alterations were associated with damages of myelin, axonal and cellular organelles. Normobaric-hyperoxia inducts mitochondria oxidative stress (MOS) and the subsequent rise of ROS causes variety of ultrastructure morphological pathological alterations in the organelles of cortical brain cells.
doi:10.4067/s0717-95022018000401310 fatcat:acko5d7g6fgt5b3gajat6r5nza